- Address
- PM Flow Cytometry Facility
- Princess Margaret Cancer Centre
- 610 University Avenue
- 8th Floor, Room 605
- Toronto, Ontario
- M5G 2M9
- Canada
- Contact
- Tel: 416-946-4501 ext. 5237
- pmflow@uhnresearch.ca
Our experienced staff can provide training, assisted acquisition, consultation, and data analysis upon request. To learn more about the services and how to access them, please email us at pmflow@uhnresearch.ca.
We are open to all UHN Investigators and External Clients. Access will require user registration and an introduction to the facility policies and instruments. New personnel will require training for the instruments they wish to use, and once training has been completed, the users will be provided with secure access to our facility.
For a detailed guide on how to create a new user account and access PM Flow, please refer to our manual, which you can download here.
Please read our After-Hours Policy and Policy for Sorting Cancellations.
Over
active users from 46 different labs served
Currently serving
different institutions
Over
years of combined experience
We provide cutting-edge equipment to help research communities accelerate their research.
Our facility is currently equipped with seven BD analytical flow cytometers whose configurations vary. To view more information on the number of lasers and detectors available, click show more to obtain additional details for individual instruments.
| Laser Color/Wavelength | Fluorescent Channels | Typical Fluorochromes per channel |
|---|---|---|
| UV (355nm) | 3 | BUV395/ BUV496/ BUV737 |
| Violet (405nm) | 6 | BV421/ BV510/ BV605/ BV650/ BV711/ BV786 |
| Blue (488nm) | 2 | FITC/ PerCP-Cy5.5 |
| Yellow-Green (561nm) | 4 | PE/ PE-TR/ PE-Cy5.5/ PE-Cy7 |
| Red (640nm) | 3 | APC/ AF700/ APC-Cy7 |
| Laser Color/Wavelength | Fluorescent Channels | Typical Fluorochromes per channel |
|---|---|---|
| UV (355nm) | 3 | BUV395/ BUV496/ BUV737 |
| Violet (405nm) | 6 | BV421/ BV510/ BV605/ BV650/ BV711/ BV786 |
| Blue (488nm) | 2 | FITC/ PerCP-Cy5.5 |
| Yellow-Green (561nm) | 4 | PE/ PE-TR/ PE-Cy5.5/ PE-Cy7 |
| Red (640nm) | 3 | APC/ AF700/ APC-Cy7 |
| Laser Color/Wavelength | Fluorescent Channels | Typical Fluorochromes per channel |
|---|---|---|
| Violet (405nm) | 3 | BV421/ BV510/ BV605 |
| Blue (488nm) | 2 | FITC/ PerCP-Cy5.5 |
| Green (543nm) | 5 | PE/ PE-TR/ PE-Cy5/ PE-Cy5.5/ PE-Cy7 |
| Red (640nm) | 3 | APC/ AF700/ APC-Cy7 |
| Laser Color/Wavelength | Fluorescent Channels | Typical Fluorochromes |
|---|---|---|
| Violet (405nm) | 4 | BV421/ BV510/ BV605/ BV650 |
| Blue (488nm) | 2 | FITC/ PerCP or PerCP-Cy5.5 |
| Red (633nm) | 3 | APC/ AF700/ APC-Cy7 |
| Yellow | 4 | PE/ PETR/ PECY5 or PECY5.5/ PEcy7 |
| Laser Color/Wavelength | Fluorescent Channels | Typical Fluorochromes |
|---|---|---|
| Violet (405nm) | 4 | BV421/ BV510/ BV605/ BV650 |
| Blue (488nm) | 2 | FITC/ PerCP or PerCP-Cy5.5 |
| Red (633nm) | 3 | APC/ AF700/ APC-Cy7 |
| Yellow | 4 | PE/ PETR/ PECY5 or PECY5.5/ PEcy7 |
| Laser Color/Wavelength | Spectral Channels | Fluors/Channel* |
|---|---|---|
| 355 | 36 | 8 |
| 405 | 35 | 10 |
| 488 | 32 | 7 |
| 561 | 26 | 8 |
| 637 | 19 | 5 |
| Total | 148 | 38 |
* Fluorophore choices are only limited by the excitation wavelength and the extent of their spectral overlap (i.e., breadth of their spectral emission curves). The numbers in the table represent typical numbers for traditional fluorophore combinations. An even greater number of detectable fluors/channel can be achieved when using novel fluors with narrower emission peaks.
Our facility is currently equipped with 3 active high-speed sorters, capable of sorting cells at impressive rates. To view additional details click show more to obtain more regarding specific instruments.
| Laser Color/Wavelength | Fluorescent Channels | Typical Fluorochromes per channel |
|---|---|---|
| Violet (405nm) | 5 | BV421/ BV510/ BV605/ BV650/ BV711 |
| Blue (488nm) | 2 | FITC/ PerCP-Cy5.5 |
| Green (561nm) | 4 | PE/ PE-TR/ PE-Cy5.5/ PE-Cy7 |
| Red (640nm) | 3 | APC/ AF700/ APC-Cy7 |
| Laser Color/Wavelength | Fluorescent Channels | Typical Fluorochromes per channel |
|---|---|---|
| Violet (405nm) | 5 | BV421/ BV510/ BV605/ BV650/ BV711 |
| Blue (488nm) | 2 | FITC/ PerCP-Cy5.5 |
| Green (561nm) | 4 | PE/ PE-TR/ PE-Cy5.5/ PE-Cy7 |
| Red (640nm) | 3 | APC/ AF700/ APC-Cy7 |
| Laser Color/Wavelength | Fluorescent Channels | Typical Fluorochromes per channel |
|---|---|---|
| Violet (405nm) | 2 | BV421/ BV510 |
| Blue (488nm) | 6 | FITC/ PE/ PE-TR/ PE-Cy5/ PE-Cy5.5/ PE-Cy7 |
| Red (640nm) | 3 | APC/ AF700/ APC-Cy7 |
| Laser Color/Wavelength | Fluorescent Channels | Typical Fluorochromes per channel |
|---|---|---|
| UV (355nm) | 3 | BUV395/ BUV496/ BUV737 |
| Violet (405nm) | 6 | BV421/ BV510/ BV605/ BV650/ BV711/ BV786 |
| Blue (488nm) | 2 | FITC/ PerCP-Cy5.5 |
| Yellow-Green (561nm) | 4 | PE/ PE-TR/ PE-Cy5.5/ PE-Cy7 |
| Red (640nm) | 3 | APC/ AF700/ APC-Cy7 |
| Service | Instruments | Internal (Hourly) |
External Academic
(Hourly) |
External Corporate
(Hourly) |
|---|---|---|---|---|
|
Flow Analysis
(unaided) |
BD FACS Canto II* | $40.00 | $48.00 | $80.00 |
|
Flow Analysis
(unaided) |
BD Fortessa1, BD Fortessa2, BD Fortessa X20 | $40.00 | $48.00 | $80.00 |
|
Flow Analysis
(unaided) |
Cytoflex S1, Cytoflex S2, Cytoflex LX* | $40.00 | $48.00 | $80.00 |
|
Flow Analysis
(unaided) |
Sony ID7000 | $50.00 | $60.00 | $100.00 |
|
Cell sorting
(peak hours) |
Astrios 1, Astrios2, Astrios3, BD FACS Aria III* | $90.00 | $108.00 | $180.00 |
|
Cell sorting
(after hours) |
Astrios 1, Astrios2, Astrios3, BD FACS Aria III* | $135.00 | $162.00 | $270.00 |
|
Cell sorting
(peak hours) |
BD Fusion | $90.00 | $108.00 | $180.00 |
|
Cell sorting
(after hours) |
BD Fusion | $135.00 | $162.00 | $270.00 |
| Magnetic Cell Separator (unaided) | AutoMacs Pro | $40.00 | $48.00 | $80.00 |
|
Assisted acquisition, training
(peak hours) |
All instruments | $85.00 | $102.00 | $170.00 |
* Only available at PM site
** Only available at KDT site
Please visit Resources to see archived news items, previous webinars, and equipment specs via the below link.
PM Flow, along with all other UHN Core facilities, can be accessed by any researcher, regardless of organization, through our Customer Management site ( Stratocore PPMS ) here. Create a free account to communicate directly with the facility and submit quotation requests for your work. Please see our brief help documents for Login and Booking .
Scheduling a meeting with us to discuss your research and goals is the best way for us to ensure the optimal technology and equipment is applied to your project. Please contact us for a free consultation by phone at (416) 946-4501 , ext. 5237 (or 16-5237 if calling from within UHN) or by e-mail at: pmflow@uhnresearch.ca
Trained users can book our analyzers online by accessing our Customer Management site Stratocore PPMS. For a detailed guide on how to create a new user account and access PM Flow, please refer to our manual, which you can download here.
Users can book training online by accessing our Customer Management site ( Stratocore PPMS ) here. Sorting appointments can be made by contacting our staff directly by phone at (416) 946-4501 , ext. 5237 (or 16-5237 if calling from within UHN) or by e-mail at: pmflow@uhnresearch.ca.
We are located on the 8th floor of the Princess Margaret Cancer Centre (office: 8-605) at 610 University Avenue, Toronto, ON, M5G 2M9.
Our operational and biosafety policies are available to registered users through our Customer Management site ( Stratocore PPMS ) here.