- Address
- PM Flow Cytometry Facility
- Princess Margaret Cancer Centre
- 610 University Avenue
- 8th Floor, Room 605
- Toronto, Ontario
- M5G 2M9
- Canada
- Contact
- Tel: 416-946-4501 ext. 5237
- pmflow@uhnresearch.ca
Resources
2024
▸ Using the SONY ID7000 to maximize your cytometry
capabilities and workflow
February 23, 2024
Using the SONY ID7000 to maximize your cytometry capabilities and workflow
Date and Time:
Wednesday, February 21, 2024
11:00 EST
In-person: PMH room 6-604
Description: Can you standardize the
ID7000 between users / instruments / longitudinal
studies?
Can you utilize your background Autofluorescence as a
novel parameter?
Can you avoid creating single stains regardless of a bead
or a cell?
What is the benefit of having up to 3x more detectors?
▸ Revealing cellular diversity with the power of spectral
cytometry
February 05, 2024
Revealing cellular diversity with the power of spectral cytometry
Date and Time:
Wednesday, February 14, 2024
8:00 PST | 11:00 EST | 16:00 GMT | 17:00 CET
Speaker: Robert Balderas, Vice President
Biological Sciences, BD Biosciences
Description: This webcast will review
the principles and concepts of spectral flow cytometry,
describe current technology innovations, and showcase some
of the exciting discoveries made possible by spectral flow
cytometry, while also discussing practical tips and best
practices for researchers who want to adopt this
technology.
2023
▸ BD Horizon NEXT coming to Toronto - September 12, 2023
August 10, 2023
BD Horizon NEXT coming to Toronto!
September 12, 2023, 10:00 am - 4:30 pm
This is the latest version of our groundbreaking Horizon Tour that helped flow cytometry users better grasp panel design principles toward cleaner data generation. This time, our event touches on elements of spectral panel design, deep immunophenotyping and sorting, spectral sorting and imaging with the BD FACSDiscover(TM) S8 as well as some data analysis aspects for high parameter assays.
▸ 46th Annual Course in Cytometry - San Diego August 12 -
August 18, 2023
March 17, 2023
46th Annual Course in Cytometry - San Diego
August 12 - August 18, 2023
San Diego is excited to be the newest location for this long running series of week-long workshops, which provide state-of-the-art information on cutting-edge topics in cytometry.
2022
▸ Deep Science Seminar April 13th, 2022
March 17, 2022
Deep Science Seminar
April 13th, 2022 at 1 p.m. ET
This presentation will highlight how the combined use of high-parameter analytical flow cytometry and advanced single-cell multiomic tools (proteomic and genomic) can help define functional murine NK cell subsets.
▸ Webinar: High-Dimensional High-Throughput Rare Event
Immunophenotyping
February 17, 2022
Webinar: High-Dimensional High-Throughput Rare Event Immunophenotyping
This webinar will provide insights for researchers who
want to learn how spectral technology, such as that on the
ID7000, can increase flexibility when using greater than
20 markers in multicolor flow cytometry experiments.
Wednesday, February 23, 2022, 8:00 am PST | 11:00 am
EST
2021
▸ Holiday Hours December 20, 2021
December 20, 2021
Holiday Hours
The PM Flow cytometry facility will be on weekend/holiday access from Saturday December 25 to Tuesday December 28th and from Saturday January 1st to Monday January 3rd, 2022, inclusive.
▸ Changes to sorting fees starting November 8th, 2021
November 1, 2021
Changes to sorting fees starting NOVEMBER 8th, 2021
1) Base rate of Sorts on Fusion will be charged at 85/h
(corporate and after-hours fees will be affected
proportionally). We will maintain the $75/h rate on all
Astrios sorters.
2) Saturday sort requests will be charged at a premium
1.5X (after hours rate). If you have any questions or
concerns about this update, please contact us at
pmflow@uhnresearch.ca.
▸ REMEMBER TO LOG OUT OF WINDOWS ON OUR COMPUTERS!
June 22, 2021
REMEMBER TO LOG OUT OF WINDOWS ON OUR COMPUTERS!
In reviewing the user activity on the X-20 since we implemented the new Windows sign-in method, it has come to our attention that some of you are forgetting to log out at the end of your analyzer sessions...
▸ Decommissioning CantoII and XDP
June 22, 2021
Decommissioning CantoII and XDP
PM Flow continues to update its instruments to better serve the research community. It is time to retire our older instruments. The CantoII and XDP will be decommissioned, and no longer available for use as of June 22 except for data retrieval. Please back up your data ASAP. If you have any questions, please, do not hesitate to contact us at pmflow@uhnresearch.ca.
▸ CytoFLEX webinar July 14th
June 22, 2021
CytoFLEX webinar July 14th
Join us on July 14 th at 12:30 pm EST and learn how to
Drive Your Flow Forward with the CytoFLEX flow cytometry
platform.
We are excited to invite you to an hour of learning and
engagement with your Beckman Coulter Life Sciences team,
Natalia Fedianina...
▸ Introducing the SONY ID7000
June 22, 2021
Introducing the SONY ID7000
Our new Sony ID7000 Spectral Analyzer has arrived and is
being installed in Room 8-516!
User training will be available starting on August 16th,
2021. Please submit training requests to
pmflow@uhnresearch.ca.
▸ Cytoflex S1 relocated to Room 10-609!
June 10, 2021
Cytoflex S1 relocated to Room 10-609!
Please be advised that the CytoFLEX S1 instrument has been relocated to Room 10-609 on the 10th floor of our facility in order to make room downstairs for the new Sony analyzers. This means that both CytoFLEX instruments (i.e., the S1 and S2) are in the same room now.
▸ ATTENTION ALL PM FLOW USERS! May 05, 2021
May 05, 2021
ATTENTION ALL PM FLOW USERS!
New! Starting from May 10, all users are required to record the 15 mins SIT and 1 min sample waste line cleaning procedures on the last tube of their experiment under BDFACS DIVA.
▸ Ask the Experts May 05, 2021
May 05, 2021
Ask the Experts
Join us on Thursday, May 13, 2021 8 a.m. PST for our Global Webinar - Ask the Experts: An Optimized Method for Accurate Spread Measurement and Its Use in Panel Design
▸ ATTENTION ALL FLOWJO USERS! April 22, 2021
April 22, 2021
ATTENTION ALL FLOWJO USERS!
FlowJo licenses are renewed annually on June 1st.
▸ NEW Fusion cancellation policy
March 23, 2021
NEW Fusion cancellation policy
As of April 1, 2021, the PMFlow facility will require 48 hours-notice to cancel sorts on the Fusion without penalty.
▸ Virtual Flow Cytometry Data Analysis Course Spring 2021
January 25, 2021
Virtual Flow Cytometry Data Analysis Course Spring 2021
It's an online flow cytometry data analysis course being organized from March 8-11 by the Royal Microscopy Society in the UK. The cost of registration is 300 British pounds.
2020
▸ Instrument Relocation to Improve Safe Access
June 5, 2020
Instrument Relocation to Improve Safe Access
We have recently relocated some of our analytical instruments in order to allow all instruments to be accessible at any time while maintaining appropriate physical distancing.
▸ ATTENTION ALL PMFlow USERS June 1, 2020
June 1, 2020
ATTENTION ALL PMFlow USERS
Things you need to know about PMFlow Research Restart June 1, 2020
▸ ATTENTION ALL FLOWJO USERS! May 20, 2020
May 20, 2020
ATTENTION ALL FLOWJO USERS!
FlowJo Analysis Software Migrates to Portal. Current Licenses expire May 31, 2020
2019
▸ Stratocore calendar
Oct 25, 2019
Stratocore calendar
The flow cytometry Calcium booking calender is no longer in use. The new Booking-Calendar application from Stratocore is live today!
▸ New Beckman Coulter Cytoflex Analyzers have arrived
Oct 25, 2019
New Beckman Coulter Cytoflex Analyzers have arrived
The first of our new Beckman Coulter Cytoflex Analyzers has arrived and will be available to our users in November. This instrument is equipped with 3 lasers (VBR) and will be capable of analyzing 13 fluorescent parameters simultaneously. Feel free to contact us for additional information and to inquire about training.
▸ FCS Express 7 Site License Demo
Sep 30, 2019
FCS Express 7 Site License Demo
Discover how to save time with your standard flow data analysis using UHN's FCS Express site license!
BD Biosciences
Beckman Coulter
Cytoflex:
MoFlo Astrios:
Sony
▸ Mar 6 - 2022: Rare event phenotyping on the Sony ID7000
Note: Users are required to fill in registration information in order to access the video.
Analyzers
▸ Fortessa 1
| Laser Name | Wavelength | Power | Detector | Channel | Mirror | Filter | Parameter | Test Parameter |
|---|---|---|---|---|---|---|---|---|
| Blue | 488 | 50 | A | 1 | 685 LP | 710/50 BP | PerCP-Cy5_5 | PerCP-Cy5_5 |
| B | 2 | 505 LP | 530/30 BP | FITC | FITC | |||
| C | 3 | 488/10 BP | SSC | |||||
| Violet | 405 | 50 | A | 4 | 750 LP | 780/60 BP | BV786 | BV786 |
| B | 5 | 690 LP | 710/50 BP | BV711 | BV711 | |||
| C | 6 | 630 LP | 660/20 BP | BV650 | BV650 | |||
| D | 7 | 595 LP | 610/20 BP | BV605 | BV605 | |||
| E | 8 | 505 LP | 525/50 BP | AmCyan | BV510 | |||
| F | 9 | 450/50 BP | Pacific Blue | BV421 | ||||
| Yel/Grn | 561 | 50 | A | 10 | 750 LP | 780/60 BP | PE-Cy7 | PE-Cy7 |
| B | 11 | 685 LP | 710/50 BP | PE-Cy5_5 | PE-Cy5_5 | |||
| C | 12 | 600 LP | 610/20 BP | PE-Texas Red | PE-Texas Red | |||
| D | 13 | 586/15 BP | PE | PE | ||||
| UV | 355 | 20 | A | 14 | 690 LP | 740/35 BP | BUV737 | BUV737 |
| B | 15 | 490 LP | 515/30 BP | BUV496 | BUV496 | |||
| C | 16 | 379/28 BP | BUV395 | BUV395 | ||||
| Red | 640 | 40 | A | 17 | 750 LP | 780/60 BP | APC-Cy7 | APC-Cy7 |
| B | 18 | 690 LP | 710/50 BP | Alexa Fluor 700 | APC-R700 | |||
| C | 19 | 670/14 BP | APC | APC |
▸ Fortessa 2
| Laser Name | Wavelength | Power | Detector | Channel | Mirror | Filter | Parameter | Test Parameter |
|---|---|---|---|---|---|---|---|---|
| Blue | 488 | 50 | A | 1 | 685 LP | 710/50 BP | PerCP-Cy5_5 | PerCP-Cy5_5 |
| B | 2 | 505 LP | 530/30 BP | FITC | FITC | |||
| C | 3 | 488/10 BP | SSC | |||||
| Violet | 405 | 50 | A | 4 | 750 LP | 780/60 BP | BV786 | BV786 |
| B | 5 | 690 LP | 710/50 BP | BV711 | BV711 | |||
| C | 6 | 630 LP | 660/20 BP | BV650 | BV650 | |||
| D | 7 | 595 LP | 610/20 BP | BV605 | BV605 | |||
| E | 8 | 505 LP | 525/50 BP | AmCyan | BV510 | |||
| F | 9 | 450/50 BP | Pacific Blue | BV421 | ||||
| Yel/Grn | 561 | 50 | A | 10 | 750 LP | 780/60 BP | PE-Cy7 | PE-Cy7 |
| B | 11 | 685 LP | 710/50 BP | PE-Cy5_5 | PE-Cy5_5 | |||
| C | 12 | 600 LP | 610/20 BP | PE-Texas Red | PE-Texas Red | |||
| D | 13 | 586/15 BP | PE | PE | ||||
| UV | 355 | 20 | A | 14 | 690 LP | 740/35 BP | BUV737 | BUV737 |
| B | 15 | 490 LP | 515/30 BP | BUV496 | BUV496 | |||
| C | 16 | 379/28 BP | BUV395 | BUV395 | ||||
| Red | 640 | 40 | A | 17 | 750 LP | 780/60 BP | APC-Cy7 | APC-Cy7 |
| B | 18 | 690 LP | 710/50 BP | Alexa Fluor 700 | APC-R700 | |||
| C | 19 | 670/14 BP | APC | APC |
▸ X-20
| Laser Name | Wavelength | Power | Detector | Channel | Mirror | Filter | Parameter | Test Parameter |
|---|---|---|---|---|---|---|---|---|
| Blue | 488 | 100 | A | 3 | 655 LP | 685/35 BP | PerCP-Cy5-5 | PerCP-Cy5_5 |
| B | 2 | 505 LP | 525/50 BP | FITC | FITC | |||
| C | 1 | 488/10 BP | SSC | |||||
| Grn | 532 | 150 | A | 8 | 750 LP | 780/60 BP | PE-Cy7 | PE-Cy7 |
| B | 7 | 685 LP | 710/50 BP | PE-Cy5.5 | PE-Cy5.5 | |||
| C | 6 | 630 LP | 660/20 BP | PE-Cy5 | PE-Cy5 | |||
| D | 5 | 600 LP | 610/20 BP | PE-Texas Red | PE-Texas Red | |||
| E | 4 | 575/25 BP | PE | PE | ||||
| Red | 640 | 40 | A | 11 | 750 LP | 780/60 BP | APC-Cy7 | APC-Cy7 |
| B | 10 | 685 LP | 710/50 BP | APC-Alexa 700 | APC-R700 | |||
| C | 9 | 670/30 BP | APC | APC | ||||
| Violet | 405 | 50 | A | 14 | 600 LP | 610/20 BP | BV605 | BV605 |
| B | 13 | 505 LP | 525/50 BP | BV510 | BV510 | |||
| C | 12 | 450/50 BP | BV421 | BV421 |
▸ Cytoflex S1
| Laser Name | Wavelength | Power | Channel | Mirror | Filter | Parameter |
|---|---|---|---|---|---|---|
| Red | 660 | 1 | R763LP | 763/43 | AC7 | |
| 2 | R660LP | 660/10 BP | APC | |||
| 3 | R712LP | 712/25 BP | A700 | |||
| Grn | 561 | 4 | Y610SP | 610/20 | PE-TR | |
| 5 | Y763LP | 763/43 | PC7 | |||
| 6 | Y585SP | 585/42 | PE | |||
| 7 | Y710LP | 710/50 | PE-Cy5.5 | |||
| Blue | 488 | 8 | B690SP | 690/50 BP | PerCP-Cy5-5 | |
| 9 | B525SP | 525/40 BP | FITC | |||
| 488/8 BP | SSC | |||||
| Violet | 405 | 10 | V450SP | 450/45 | BV421 | |
| 11 | V525SP | 525/40 | BV510 | |||
| 12 | V610LP | 610/20 | BV605 | |||
| 13 | V660LP | 660/10 | BV650 |
▸ Cytoflex S2
| Laser Name | Wavelength | Power | Channel | Mirror | Filter | Parameter |
|---|---|---|---|---|---|---|
| Red | 660 | 1 | R763LP | 763/43 | AC7 | |
| 2 | R660LP | 660/10 BP | APC | |||
| 3 | R712LP | 712/25 BP | A700 | |||
| Grn | 561 | 4 | Y610SP | 610/20 | PE-TR | |
| 5 | Y763LP | 763/43 | PC7 | |||
| 6 | Y585SP | 585/42 | PE | |||
| 7 | Y710LP | 710/50 | PE-Cy5.5 | |||
| Blue | 488 | 8 | B690SP | 690/50 BP | PerCP-Cy5-5 | |
| 9 | B525SP | 525/40 BP | FITC | |||
| 488/8 BP | SSC | |||||
| Violet | 405 | 10 | V450SP | 450/45 | BV421 | |
| 11 | V525SP | 525/40 | BV510 | |||
| 12 | V610LP | 610/20 | BV605 | |||
| 13 | V660LP | 660/10 | BV650 |
Sorters
▸ Fusion
| Laser Name | Wavelength | Power | Detector | Channel | Mirror | Filter | Parameter | Test Parameter |
|---|---|---|---|---|---|---|---|---|
| Blue | 488 | 50 | A | 1 | 685 LP | 710/50 BP | PerCP-Cy5_5 | PerCP-Cy5_5 |
| B | 2 | 505 LP | 530/30 BP | FITC | FITC | |||
| C | 3 | 488/10 BP | SSC | |||||
| Violet | 405 | 50 | A | 4 | 750 LP | 780/60 BP | BV786 | BV786 |
| B | 5 | 690 LP | 710/50 BP | BV711 | BV711 | |||
| C | 6 | 630 LP | 660/20 BP | BV650 | BV650 | |||
| D | 7 | 595 LP | 610/20 BP | BV605 | BV605 | |||
| E | 8 | 505 LP | 525/50 BP | AmCyan | BV510 | |||
| F | 9 | 450/50 BP | Pacific Blue | BV421 | ||||
| Yel/Grn | 561 | 50 | A | 10 | 750 LP | 780/60 BP | PE-Cy7 | PE-Cy7 |
| B | 11 | 685 LP | 710/50 BP | PE-Cy5_5 | PE-Cy5_5 | |||
| C | 12 | 600 LP | 610/20 BP | PE-Texas Red | PE-Texas Red | |||
| D | 13 | 586/15 BP | PE | PE | ||||
| UV | 355 | 20 | A | 14 | 690 LP | 740/35 BP | BUV737 | BUV737 |
| B | 15 | 490 LP | 515/30 BP | BUV496 | BUV496 | |||
| C | 16 | 379/28 BP | BUV395 | BUV395 | ||||
| Red | 640 | 40 | A | 17 | 750 LP | 780/60 BP | APC-Cy7 | APC-Cy7 |
| B | 18 | 690 LP | 710/50 BP | Alexa Fluor 700 | APC-R700 | |||
| C | 19 | 670/14 BP | APC | APC |
▸ Astrios 1
| Laser Name | Wavelength (nm) | Power (mW) | Detector | Mirror | Filter | Fluors |
|---|---|---|---|---|---|---|
| Violet | 405 | 55 | A | 418 DLP | 405/10 | V-SSC |
| B | M | 448/59 | BV421 | |||
| C | 506 DLP | 525/50 | BV510 | |||
| D | 635 DLP | 615/20 | BV605 | |||
| E | 681 DLP | 661/20 | BV650 | |||
| F | 681 DLP | 710/40 | BV710 | |||
| Blue | 488 | 200 | A | M | 488/6 | B-SSC |
| B | 495 DLP | 525/50 | FITC, AlexaFluor 488, GFP | |||
| C | M | 698/70 | PerCP-Cy5.5, 7-AAD | |||
| Green | 561 | 200 | A | M | 579/16 | TRITC, mRFP, PE, tdTomato |
| B | 595 DLP | 641/20 | mCherry, PI, PE-Cy5, Texas Red | |||
| C | 757DLP | 692/75 | 7-AAD, PE-cy5.5 | |||
| D | M | 795/70 | PE-Cy7 | |||
| Red | 640 | 100 | A | M | 642/10 | R-SSC |
| B | 650 DSP | 671/30 | APC, AlexaFluor 647 | |||
| C | 695 DSP | 722/44 | AF700 | |||
| D | 755 DSP | 795/70 | APC-Cy7, AlexaFluor 750 |
▸ Astrios 2
| Laser Name | Wavelength (nm) | Power (mW) | Detector | Mirror | Filter | Fluors |
|---|---|---|---|---|---|---|
| Violet | 405 | 55 | A | 418 DLP | 405/10 | V-SSC |
| B | M | 448/59 | BV421 | |||
| C | 506 DLP | 525/50 | BV510 | |||
| D | 635 DLP | 615/20 | BV605 | |||
| E | 681 DLP | 661/20 | BV650 | |||
| F | 681 DLP | 710/40 | BV710 | |||
| Blue | 488 | 200 | A | M | 488/6 | B-SSC |
| B | 495 DLP | 525/50 | FITC, AlexaFluor 488, GFP | |||
| C | M | 698/70 | PerCP-Cy5.5, 7-AAD | |||
| Green | 561 | 200 | A | M | 579/16 | TRITC, mRFP, PE, tdTomato |
| B | 595 DLP | 641/20 | mCherry, PI, PE-Cy5, Texas Red | |||
| C | 757DLP | 692/75 | 7-AAD, PE-cy5.5 | |||
| D | M | 795/70 | PE-Cy7 | |||
| Red | 640 | 100 | A | M | 642/10 | R-SSC |
| B | 650 DSP | 671/30 | APC, AlexaFluor 647 | |||
| C | 695 DSP | 722/44 | AF700 | |||
| D | 755 DSP | 795/70 | APC-Cy7, AlexaFluor 750 |