- Address
- PM Flow Cytometry Facility
- Princess Margaret Cancer Centre
- 610 University Avenue
- 8th Floor, Room 605
- Toronto, Ontario
- M5G 2M9
- Canada
- Contact
- Tel: 416-946-4501 ext. 5237
- pmflow@uhn.ca
Laser Name | Wavelength | Power | Detector | Channel | Mirror | Filter | Parameter | Test Parameter |
---|---|---|---|---|---|---|---|---|
Blue | 488 | 50 | A | 1 | 685 LP | 710/50 BP | PerCP-Cy5_5 | PerCP-Cy5_5 |
B | 2 | 505 LP | 530/30 BP | FITC | FITC | |||
C | 3 | 488/10 BP | SSC | |||||
Violet | 405 | 50 | A | 4 | 750 LP | 780/60 BP | BV786 | BV786 |
B | 5 | 690 LP | 710/50 BP | BV711 | BV711 | |||
C | 6 | 630 LP | 660/20 BP | BV650 | BV650 | |||
D | 7 | 595 LP | 610/20 BP | BV605 | BV605 | |||
E | 8 | 505 LP | 525/50 BP | AmCyan | BV510 | |||
F | 9 | 450/50 BP | Pacific Blue | BV421 | ||||
Yel/Grn | 561 | 50 | A | 10 | 750 LP | 780/60 BP | PE-Cy7 | PE-Cy7 |
B | 11 | 685 LP | 710/50 BP | PE-Cy5_5 | PE-Cy5_5 | |||
C | 12 | 600 LP | 610/20 BP | PE-Texas Red | PE-Texas Red | |||
D | 13 | 586/15 BP | PE | PE | ||||
UV | 355 | 20 | A | 14 | 690 LP | 740/35 BP | BUV737 | BUV737 |
B | 15 | 490 LP | 515/30 BP | BUV496 | BUV496 | |||
C | 16 | 379/28 BP | BUV395 | BUV395 | ||||
Red | 640 | 40 | A | 17 | 750 LP | 780/60 BP | APC-Cy7 | APC-Cy7 |
B | 18 | 690 LP | 710/50 BP | Alexa Fluor 700 | APC-R700 | |||
C | 19 | 670/14 BP | APC | APC |
Laser Name | Wavelength | Power | Detector | Channel | Mirror | Filter | Parameter | Test Parameter |
---|---|---|---|---|---|---|---|---|
Blue | 488 | 50 | A | 1 | 685 LP | 710/50 BP | PerCP-Cy5_5 | PerCP-Cy5_5 |
B | 2 | 505 LP | 530/30 BP | FITC | FITC | |||
C | 3 | 488/10 BP | SSC | |||||
Violet | 405 | 50 | A | 4 | 750 LP | 780/60 BP | BV786 | BV786 |
B | 5 | 690 LP | 710/50 BP | BV711 | BV711 | |||
C | 6 | 630 LP | 660/20 BP | BV650 | BV650 | |||
D | 7 | 595 LP | 610/20 BP | BV605 | BV605 | |||
E | 8 | 505 LP | 525/50 BP | AmCyan | BV510 | |||
F | 9 | 450/50 BP | Pacific Blue | BV421 | ||||
Yel/Grn | 561 | 50 | A | 10 | 750 LP | 780/60 BP | PE-Cy7 | PE-Cy7 |
B | 11 | 685 LP | 710/50 BP | PE-Cy5_5 | PE-Cy5_5 | |||
C | 12 | 600 LP | 610/20 BP | PE-Texas Red | PE-Texas Red | |||
D | 13 | 586/15 BP | PE | PE | ||||
UV | 355 | 20 | A | 14 | 690 LP | 740/35 BP | BUV737 | BUV737 |
B | 15 | 490 LP | 515/30 BP | BUV496 | BUV496 | |||
C | 16 | 379/28 BP | BUV395 | BUV395 | ||||
Red | 640 | 40 | A | 17 | 750 LP | 780/60 BP | APC-Cy7 | APC-Cy7 |
B | 18 | 690 LP | 710/50 BP | Alexa Fluor 700 | APC-R700 | |||
C | 19 | 670/14 BP | APC | APC |
Laser Name | Wavelength | Power | Detector | Channel | Mirror | Filter | Parameter | Test Parameter |
---|---|---|---|---|---|---|---|---|
Blue | 488 | 100 | A | 3 | 655 LP | 685/35 BP | PerCP-Cy5-5 | PerCP-Cy5_5 |
B | 2 | 505 LP | 525/50 BP | FITC | FITC | |||
C | 1 | 488/10 BP | SSC | |||||
Grn | 532 | 150 | A | 8 | 750 LP | 780/60 BP | PE-Cy7 | PE-Cy7 |
B | 7 | 685 LP | 710/50 BP | PE-Cy5.5 | PE-Cy5.5 | |||
C | 6 | 630 LP | 660/20 BP | PE-Cy5 | PE-Cy5 | |||
D | 5 | 600 LP | 610/20 BP | PE-Texas Red | PE-Texas Red | |||
E | 4 | 575/25 BP | PE | PE | ||||
Red | 640 | 40 | A | 11 | 750 LP | 780/60 BP | APC-Cy7 | APC-Cy7 |
B | 10 | 685 LP | 710/50 BP | APC-Alexa 700 | APC-R700 | |||
C | 9 | 670/30 BP | APC | APC | ||||
Violet | 405 | 50 | A | 14 | 600 LP | 610/20 BP | BV605 | BV605 |
B | 13 | 505 LP | 525/50 BP | BV510 | BV510 | |||
C | 12 | 450/50 BP | BV421 | BV421 |
Laser Name | Wavelength | Power | Channel | Mirror | Filter | Parameter |
---|---|---|---|---|---|---|
Red | 660 | 1 | R763LP | 763/43 | AC7 | |
2 | R660LP | 660/10 BP | APC | |||
3 | R712LP | 712/25 BP | A700 | |||
Grn | 561 | 4 | Y610SP | 610/20 | PE-TR | |
5 | Y763LP | 763/43 | PC7 | |||
6 | Y585SP | 585/42 | PE | |||
7 | Y710LP | 710/50 | PE-Cy5.5 | |||
Blue | 488 | 8 | B690SP | 690/50 BP | PerCP-Cy5-5 | |
9 | B525SP | 525/40 BP | FITC | |||
488/8 BP | SSC | |||||
Violet | 405 | 10 | V450SP | 450/45 | BV421 | |
11 | V525SP | 525/40 | BV510 | |||
12 | V610LP | 610/20 | BV605 | |||
13 | V660LP | 660/10 | BV650 |
Laser Name | Wavelength | Power | Channel | Mirror | Filter | Parameter |
---|---|---|---|---|---|---|
Red | 660 | 1 | R763LP | 763/43 | AC7 | |
2 | R660LP | 660/10 BP | APC | |||
3 | R712LP | 712/25 BP | A700 | |||
Grn | 561 | 4 | Y610SP | 610/20 | PE-TR | |
5 | Y763LP | 763/43 | PC7 | |||
6 | Y585SP | 585/42 | PE | |||
7 | Y710LP | 710/50 | PE-Cy5.5 | |||
Blue | 488 | 8 | B690SP | 690/50 BP | PerCP-Cy5-5 | |
9 | B525SP | 525/40 BP | FITC | |||
488/8 BP | SSC | |||||
Violet | 405 | 10 | V450SP | 450/45 | BV421 | |
11 | V525SP | 525/40 | BV510 | |||
12 | V610LP | 610/20 | BV605 | |||
13 | V660LP | 660/10 | BV650 |
Laser Name | Wavelength | Power | Detector | Channel | Mirror | Filter | Parameter | Test Parameter |
---|---|---|---|---|---|---|---|---|
Blue | 488 | 50 | A | 1 | 685 LP | 710/50 BP | PerCP-Cy5_5 | PerCP-Cy5_5 |
B | 2 | 505 LP | 530/30 BP | FITC | FITC | |||
C | 3 | 488/10 BP | SSC | |||||
Violet | 405 | 50 | A | 4 | 750 LP | 780/60 BP | BV786 | BV786 |
B | 5 | 690 LP | 710/50 BP | BV711 | BV711 | |||
C | 6 | 630 LP | 660/20 BP | BV650 | BV650 | |||
D | 7 | 595 LP | 610/20 BP | BV605 | BV605 | |||
E | 8 | 505 LP | 525/50 BP | AmCyan | BV510 | |||
F | 9 | 450/50 BP | Pacific Blue | BV421 | ||||
Yel/Grn | 561 | 50 | A | 10 | 750 LP | 780/60 BP | PE-Cy7 | PE-Cy7 |
B | 11 | 685 LP | 710/50 BP | PE-Cy5_5 | PE-Cy5_5 | |||
C | 12 | 600 LP | 610/20 BP | PE-Texas Red | PE-Texas Red | |||
D | 13 | 586/15 BP | PE | PE | ||||
UV | 355 | 20 | A | 14 | 690 LP | 740/35 BP | BUV737 | BUV737 |
B | 15 | 490 LP | 515/30 BP | BUV496 | BUV496 | |||
C | 16 | 379/28 BP | BUV395 | BUV395 | ||||
Red | 640 | 40 | A | 17 | 750 LP | 780/60 BP | APC-Cy7 | APC-Cy7 |
B | 18 | 690 LP | 710/50 BP | Alexa Fluor 700 | APC-R700 | |||
C | 19 | 670/14 BP | APC | APC |
Laser Name | Wavelength (nm) | Power (mW) | Detector | Mirror | Filter | Fluors |
---|---|---|---|---|---|---|
Violet | 405 | 55 | A | 418 DLP | 405/10 | V-SSC |
B | M | 448/59 | BV421 | |||
C | 506 DLP | 525/50 | BV510 | |||
D | 635 DLP | 615/20 | BV605 | |||
E | 681 DLP | 661/20 | BV650 | |||
F | 681 DLP | 710/40 | BV710 | |||
Blue | 488 | 200 | A | M | 488/6 | B-SSC |
B | 495 DLP | 525/50 | FITC, AlexaFluor 488, GFP | |||
C | M | 698/70 | PerCP-Cy5.5, 7-AAD | |||
Green | 561 | 200 | A | M | 579/16 | TRITC, mRFP, PE, tdTomato |
B | 595 DLP | 641/20 | mCherry, PI, PE-Cy5, Texas Red | |||
C | 757DLP | 692/75 | 7-AAD, PE-cy5.5 | |||
D | M | 795/70 | PE-Cy7 | |||
Red | 640 | 100 | A | M | 642/10 | R-SSC |
B | 650 DSP | 671/30 | APC, AlexaFluor 647 | |||
C | 695 DSP | 722/44 | AF700 | |||
D | 755 DSP | 795/70 | APC-Cy7, AlexaFluor 750 |
Laser Name | Wavelength (nm) | Power (mW) | Detector | Mirror | Filter | Fluors |
---|---|---|---|---|---|---|
Violet | 405 | 55 | A | 418 DLP | 405/10 | V-SSC |
B | M | 448/59 | BV421 | |||
C | 506 DLP | 525/50 | BV510 | |||
D | 635 DLP | 615/20 | BV605 | |||
E | 681 DLP | 661/20 | BV650 | |||
F | 681 DLP | 710/40 | BV710 | |||
Blue | 488 | 200 | A | M | 488/6 | B-SSC |
B | 495 DLP | 525/50 | FITC, AlexaFluor 488, GFP | |||
C | M | 698/70 | PerCP-Cy5.5, 7-AAD | |||
Green | 561 | 200 | A | M | 579/16 | TRITC, mRFP, PE, tdTomato |
B | 595 DLP | 641/20 | mCherry, PI, PE-Cy5, Texas Red | |||
C | 757DLP | 692/75 | 7-AAD, PE-cy5.5 | |||
D | M | 795/70 | PE-Cy7 | |||
Red | 640 | 100 | A | M | 642/10 | R-SSC |
B | 650 DSP | 671/30 | APC, AlexaFluor 647 | |||
C | 695 DSP | 722/44 | AF700 | |||
D | 755 DSP | 795/70 | APC-Cy7, AlexaFluor 750 |