PM Flow Logo Reseach and Innovation Cores Logo

Driving research forward with state-of-the-art technology and expertise.

The Princess Margaret Flow facility offers state-of-the-art analytical flow cytometry and cell sorting services to both UHN and other local medical research organizations in the Greater Toronto Area.

Get Started
PM Flow Logo Reseach and Innovation Cores Logo

Driving research forward with state-of-the-art technology and expertise.

The Princess Margaret Flow facility offers state-of-the-art analytical flow cytometry and cell sorting services to both UHN and other local medical research organizations in the Greater Toronto Area.

Get Started

Welcome to PM Flow Cytometry Facility

Our experienced staff can provide training, assisted acquisition, consultation and data analysis upon request. To learn more about the services and how to access them, you can contact us.

Over

250

active users from 46 different labs served

Currently serving

11

different institutions

Over

50

years of combined experience

What We Offer

PM Flow offers many services for all of their users.

Analytical Flow Cytometry

+

Analytical Flow Cytometry

  • 6 Analytical Flow Cytometers may be used to quantify suspended cells, or other particles (e.g., beads), that have been previously labeled with fluorescent reagents
  • Analyze up to 18 different fluorescent parameters simultaneously at a flow rate of up to 40,000 particles/second
  • UHN personnel with After-Hours Access may use our flow cytometers 24/7
  • Visiting researchers may access our equipment during operational hours with operator assisted processing

Fluorescence Activated Cell Sorting

+

FACS

  • Dedicated operators provide high-speed cell sorting services using the 3 FACS instruments
  • Analyze up to 18 different fluorescent parameters simultaneously at a flow rate of up to 40,000 particles/second
  • Sort cells at typical speeds ranging from 4000 - 20000 events per second depending on the size of cells and/or their sensitivity to fluidic pressure.

Consultation & Data Analysis

+

Consultation & Data Analysis

  • Help designing a new flow cytometry panel or designing a new experiment.
  • Help analyzing your flow cytometry data or preparing it for publication.
  • Instructing new users on theoretical concepts and instrument operation.
  • PMflow offers our customers a FlowJo Portal Site License at a discounted price of $350 per year. This is a user-based licensing system for a popular flow analysis software For more info. please contact pmflow@uhnresearch.ca.

What We Offer

PM Flow offers many services for all of their users.

Analytical Flow Cytometry

+

Analytical Flow Cytometry

  • 6 Analytical Flow Cytometers may be used to quantify suspended cells, or other particles (e.g., beads), that have been previously labeled with fluorescent reagents
  • Analyze up to 18 different fluorescent parameters simultaneously at a flow rate of up to 40,000 particles/second
  • UHN personnel with After-Hours Access may use our flow cytometers 24/7
  • Visiting researchers may access our equipment during operational hours with operator assisted processing

Fluorescence Activated Cell Sorting

+

FACS

  • Dedicated operators provide high-speed cell sorting services using the 3 FACS instruments
  • Analyze up to 18 different fluorescent parameters simultaneously at a flow rate of up to 40,000 particles/second
  • Sort cells at typical speeds ranging from 4000 - 20000 events per second depending on the size of cells and/or their sensitivity to fluidic pressure.

Consultation & Data Analysis

+

Consultation & Data Analysis

  • Help designing a new flow cytometry panel or designing a new experiment.
  • Help analyzing your flow cytometry data or preparing it for publication.
  • Instructing new users on theoretical concepts and instrument operation.
  • PMflow offers our customers a FlowJo Portal Site License at a discounted price of $350 per year. This is a user-based licensing system for a popular flow analysis software For more info. please contact pmflow@uhnresearch.ca.

Equipment

We provide cutting-edge equipment to help research communities accelerate their research.

Analyzers

Our facility is currently equipped with seven BD analytical flow cytometers whose configurations vary. To view more information on the number of lasers and detectors available, click show more to obtain additional details for individual instruments.

▸ BD FACS Canto II (3 lasers, 8 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes
Violet (405nm) 2 BV421/ BV510
Blue (488nm) 4 FITC/ PE/ PerCP-Cy5.5/ PE-Cy7
Red (633nm) 2 APC/ APC-Cy7
▸ BD LSR Fortessa 1 (5 lasers, 17 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
UV (355nm) 2 DAPI/ Indo1
Violet (405nm) 5 BV421/ BV510/ BV605/ BV711/ BV786
Blue (488nm) 2 FITC/ PerCP-Cy5.5
Yellow-Green (561nm) 5 PE/ PE-TR/ PE-Cy5/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ BD LSR Fortessa 2 (5 lasers, 18 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
UV (355nm) 3 BUV395/ BUV496/ BUV737
Violet (405nm) 6 BV421/ BV510/ BV605/ BV650/ BV711/ BV786
Blue (488nm) 2 FITC/ PerCP-Cy5.5
Yellow-Green (561nm) 4 PE/ PE-TR/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ BD LSR X20 (4 lasers, 13 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
Violet (405nm) 3 BV421/ BV510/ BV605
Blue (488nm) 2 FITC/ PerCP-Cy5.5/td>
Green (543nm) 5 PE/ PE-TR/ PE-Cy5/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ Beckman Cytoflex 1 (4 lasers, 13 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes
Violet (405nm) 4 BV421/ BV510/ BV605/ BV650
Blue (488nm) 2 FITC/ PerCP or PerCP-Cy5.5
Red (633nm) 3 APC/ AF700/ APC-Cy7
Yellow 4 PE/ PETR/ PECY5 or PECY5.5/ PEcy7
▸ Beckman Cytoflex 2 (4 lasers, 13 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes
Violet (405nm) 4 BV421/ BV510/ BV605/ BV650
Blue (488nm) 2 FITC/ PerCP or PerCP-Cy5.5
Red (633nm) 3 APC/ AF700/ APC-Cy7
Yellow 4 PE/ PETR/ PECY5 or PECY5.5/ PEcy7

Cell Sorters

Our facility is currently equipped with 3 active high-speed sorters, capable of sorting cells at impressive rates. To view additional details click show more to obtain more regarding specific instruments.

▸ Beckman Mo-Flo Astrios 1 (4 lasers, 14 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
Violet (405nm) 5 BV421/ BV510/ BV605/ BV650/ BV711
Blue (488nm) 2 FITC/ PerCP-Cy5.5
Green (561nm) 4 PE/ PE-TR/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ Beckman Mo-Flo Astrios 2 (3 lasers, 11 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
Violet (405nm) 2 BV421/ BV510
Blue (488nm) 6 FITC/ PE/ PE-TR/ PE-Cy5/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ BD Aria Fusion (5 lasers, 18 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
UV (355nm) 3 BUV395/ BUV496/ BUV737
Violet (405nm) 6 BV421/ BV510/ BV605/ BV650/ BV711/ BV786
Blue (488nm) 2 FITC/ PerCP-Cy5.5
Yellow-Green (561nm) 4 PE/ PE-TR/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7

Fee Table

Service Instruments Internal (Hourly) External Academic
(Hourly)
External Corporate
(Hourly)
Flow Analysis
(unaided)
BD FACS Canto II $30.00 $30.00 $60.00
Flow Analysis
(unaided)
BD Fortessa1, BD Fortessa2, BD Fortessa X20 $40.00 $40.00 $80.00
Flow Analysis
(unaided)
Cytoflex $40.00 $40.00 $80.00
Cell sorting
(peak hours)
BD Fusion, Astrios 1, Astrios2, Astrios3 $75.00 $75.00 $150.00
Cell sorting
(after hours)
BD Fusion, Astrios1, Astrios2, Astrios3 $112.50 $112.50 $225.00
Assisted acquisition, training
(peak hours)
All instruments $75.00 $75.00 $150.00

Questions?

PM Flow, along with all other UHN Core facilities, can be accessed by any researcher, regardless of organization, through our Customer Management site ( Stratocore PPMS ) here. Create a free account to communicate directly with the facility and submit quotation requests for your work. Please see our brief help documents for Login and Booking .

Scheduling a meeting with us to discuss your research and goals is the best way for us to ensure the optimal technology and equipment is applied to your project. Please contact us for a free consultation by phone at (416) 946-4501 , ext. 5237 (or 16-5237 if calling from within UHN) or by e-mail at: pmflow@uhnresearch.ca

Trained users can book our analyzers online by accessing our Customer Management site ( Stratocore PPMS ) here. Sorting appointments can be made by contacting our staff directly by phone at (416) 946-4501 , ext. 5237 (or 16-5237 if calling from within UHN) or by e-mail at: pmflow@uhnresearch.ca.

Users can book training online by accessing our Customer Management site ( Stratocore PPMS ) here. Sorting appointments can be made by contacting our staff directly by phone at (416) 946-4501 , ext. 5237 (or 16-5237 if calling from within UHN) or by e-mail at: pmflow@uhnresearch.ca.

We are located on the 8th floor of the Princess Margaret Cancer Centre (office: 8-605) at 610 University Avenue, Toronto, ON, M5G 2M9.

Our operational and biosafety policies are available to registered users through our Customer Management site ( Stratocore PPMS ) here.

Recent News

Read all about what we've been up to
picture of SBAR flow poster
June 22, 2021

REMEMBER TO LOG OUT OF WINDOWS ON OUR COMPUTERS!

In reviewing the user activity on the X-20 since we implemented the new Windows sign-in method, it has come to our attention that some of you are forgetting to log out at the end of your analyzer sessions...

Read details here ▸
picture of SBAR floJo poster
June 22, 2021

Decommissioning CantoII and XDP

PM Flow continues to update its instruments to better serve the research community. It is time to retire our older instruments. The CantoII and XDP will be decommissioned, and no longer available for use as of June 22 except for data retrieval. Please back up your data ASAP. If you have any questions, please, do not hesitate to contact us at pmflow@uhnresearch.ca.

picture of SBAR floJo poster
June 22, 2021

CytoFLEX webinar July 14th

Join us on July 14 th at 12:30 pm EST and learn how to Drive Your Flow Forward with the CytoFLEX flow cytometry platform.
We are excited to invite you to an hour of learning and engagement with your Beckman Coulter Life Sciences team, Natalia Fedianina...

Read details here ▸
picture of Sony ID7000
June 22, 2021

Introducing the SONY ID7000

Our new Sony ID7000 Spectral Analyzer has arrived and is being installed in Room 8-516!
User training will be available starting on August 16th, 2021. Please submit training requests to pmflow@uhnresearch.ca.

picture of SBAR floJo poster
June 10, 2021

Cytoflex S1 relocated to Room 10-609!

Please be advised that the CytoFLEX S1 instrument has been relocated to Room 10-609 on the 10th floor of our facility in order to make room downstairs for the new Sony analyzers. This means that both CytoFLEX instruments (i.e., the S1 and S2) are in the same room now.

picture of SBAR floJo poster
May 05, 2021

ATTENTION ALL PM FLOW USERS!

New! Starting from May 10, all users are required to record the 15 mins SIT and 1 min sample waste line cleaning procedures on the last tube of their experiment under BDFACS DIVA.

May 05, 2021

Ask the Experts

Join us on Thursday, May 13, 2021 8 a.m. PST for our Global Webinar - Ask the Experts: An Optimized Method for Accurate Spread Measurement and Its Use in Panel Design

picture of SBAR floJo poster
April 22, 2021

ATTENTION ALL FLOWJO USERS!

FlowJo licenses are renewed annually on June 1st.

picture of SBAR allusers poster
March 23, 2021

NEW Fusion cancellation policy

As of April 1, 2021, the PMFlow facility will require 48 hours-notice to cancel sorts on the Fusion without penalty.

picture of moving equipment
January 25, 2021

Virtual Flow Cytometry Data Analysis Course Spring 2021

It's an online flow cytometry data analysis course being organized from March 8-11 by the Royal Microscopy Society in the UK. The cost of registration is 300 British pounds.

picture of moving equipment
June 5, 2020

Instrument Relocation to Improve Safe Access

We have recently relocated some of our analytical instruments in order to allow all instruments to be accessible at any time while maintaining appropriate physical distancing.

picture of SBAR allusers poster
June 1, 2020

ATTENTION ALL PMFlow USERS

Things you need to know about PMFlow Research Restart June 1, 2020

picture of SBAR floJo poster
May 20, 2020

ATTENTION ALL FLOWJO USERS!

FlowJo Analysis Software Migrates to Portal. Current Licenses expire May 31, 2020

picture of calendar November
Oct 25, 2019

Stratocore calendar

The flow cytometry Calcium booking calender is no longer in use. The new Booking-Calendar application from Stratocore is live today!

picture of imaging workshop poster
Oct 25, 2019

New Beckman Coulter Cytoflex Analyzers have arrived

The first of our new Beckman Coulter Cytoflex Analyzers has arrived and will be available to our users in November. This instrument is equipped with 3 lasers (VBR) and will be capable of analyzing 13 fluorescent parameters simultaneously. Feel free to contact us for additional information and to inquire about training.

picture of fcs express bar
Sep 30, 2019

FCS Express 7 Site License Demo

Discover how to save time with your standard flow data analysis using UHN’s FCS Express site license!

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