Restarting Research Responsibly Phase 1 June 1, 2020

The PM Flow Cytometry Facility (located on the 8th floor of PM Cancer Centre) will re-open to support Research Restart Phase 1 for approved research plans and essential on-site research activities on June 1, 2020.

  • PM flow staff will be onsite during the Phase 1 mandated shifts Mon-Sat 7am-8pm to support your research needs. Please, contact us to book sorts, consult or answer any questions by email at pmflow@uhnresearch.ca
  • All instrument calendars will begin to allow instrument reservations under a new restricted 2 step process on May 28, 2020.
    º  Users will request bookings on the calendar
    º  PMFlowStaff will confirm booking with a secondary authorization step
  • Instrument bookings will be restricted based on the requirement for physical distancing. Instruments in close proximity will be mutually excluded for booking.
  • PM Flow is working to move instruments to new spaces to increase access to all instruments at one time. Stay tuned for further announcements or refer to new instrument location alerts when booking.
  • Any issues related to prioritization of research needs can be addressed by contacting the PM Flow manager, Arda Shahinian at arda.shahinian@uhnresearch.ca directly for coordination of instrument use or support.
  • All training and assisted acquisitions are suspended. We will be offering full service acquisitions to support your research needs until physical distancing requirements are eased.
  • Each user is required to clean all areas of the workspace including but not limited to benches, instrument controls, keyboards, chairs etc. after instrument use. Please book accordingly to allow ample time for these mandatory safety measures.
  • Facility staff will thoroughly clean the work areas between shifts.
  • Equipment will be maintained on a regular basis to ensure proper operation of instruments.
  • Anyone found using the instruments without prior booking or research restart approval will be charged 5X the regular fee and will be reported to the UHN executive for non-compliance.
For more information regarding Covid-19 Research Restart click here (internal connection required).
Be part of the solution by staying informed and limiting on-site research activity according to the mandated guidelines, including self-monitoring, physical distancing, mandatory masking and PPE requirements.
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Announcement

The PM Flow Cytometry Facility (located on the 8th floor of PM Cancer Centre) will re-open to support Research Restart Phase 1 for approved research plans and essential on-site research activities on June 1, 2020 .

  • PM flow staff will be onsite during the Phase 1 mandated shifts Mon-Sat 7am-8pm to support your research needs. Please, contact us to book sorts, consult or answer any questions by email at pmflow@uhnresearch.ca
  • Any issues related to prioritization of research needs can be addressed by contacting the PM Flow manager, Arda Shahinian at arda.shahinian@uhnresearch.ca directly for coordination of instrument use or support.
For more information regarding Covid-19 Research Restart click here (internal connection required).

Welcome to PM Flow Cytometry Facility

Our experienced staff can provide training, assisted acquisition, consultation and data analysis upon request. To learn more about the services and how to access them, you can contact us.

Over

250

active users from 46 different labs served

Currently serving

11

different institutions

Over

50

years of combined experience

What We Offer

PM Flow offers many services for all of their users.

Analytical Flow Cytometry

+

Analytical Flow Cytometry

  • 6 Analytical Flow Cytometers may be used to quantify suspended cells, or other particles (e.g., beads), that have been previously labeled with fluorescent reagents
  • Analyze up to 18 different fluorescent parameters simultaneously at a flow rate of up to 40,000 particles/second
  • UHN personnel with After-Hours Access may use our flow cytometers 24/7
  • Visiting researchers may access our equipment during operational hours with operator assisted processing

Fluorescence Activated Cell Sorting

+

FACS

  • Dedicated operators provide high-speed cell sorting services using the 3 FACS instruments
  • Analyze up to 18 different fluorescent parameters simultaneously at a flow rate of up to 40,000 particles/second
  • Sort cells at typical speeds ranging from 4000 - 20000 events per second depending on the size of cells and/or their sensitivity to fluidic pressure.

Consultation & Data Analysis

+

Consultation & Data Analysis

  • Help designing a new flow cytometry panel or designing a new experiment.
  • Help analyzing your flow cytometry data or preparing it for publication.
  • Instructing new users on theoretical concepts and instrument operation.

What We Offer

PM Flow offers many services for all of their users.

Analytical Flow Cytometry

+

Analytical Flow Cytometry

  • 6 Analytical Flow Cytometers may be used to quantify suspended cells, or other particles (e.g., beads), that have been previously labeled with fluorescent reagents
  • Analyze up to 18 different fluorescent parameters simultaneously at a flow rate of up to 40,000 particles/second
  • UHN personnel with After-Hours Access may use our flow cytometers 24/7
  • Visiting researchers may access our equipment during operational hours with operator assisted processing

Fluorescence Activated Cell Sorting

+

FACS

  • Dedicated operators provide high-speed cell sorting services using the 3 FACS instruments
  • Analyze up to 18 different fluorescent parameters simultaneously at a flow rate of up to 40,000 particles/second
  • Sort cells at typical speeds ranging from 4000 - 20000 events per second depending on the size of cells and/or their sensitivity to fluidic pressure.

Consultation & Data Analysis

+

Consultation & Data Analysis

  • Help designing a new flow cytometry panel or designing a new experiment.
  • Help analyzing your flow cytometry data or preparing it for publication.
  • Instructing new users on theoretical concepts and instrument operation.

Equipment

We provide cutting-edge equipment to help research communities accelerate their research.

Analyzers

Our facility is currently equipped with seven BD analytical flow cytometers whose configurations vary. To view more information on the number of lasers and detectors available, click show more to obtain additional details for individual instruments.

▸ BD FACS Canto II (3 lasers, 8 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes
Violet (405nm) 2 BV421/ BV510
Blue (488nm) 4 FITC/ PE/ PerCP-Cy5.5/ PE-Cy7
Red (633nm) 2 APC/ APC-Cy7
▸ BD LSR Fortessa 1 (5 lasers, 17 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
UV (355nm) 2 DAPI/ Indo1
Violet (405nm) 5 BV421/ BV510/ BV605/ BV711/ BV786
Blue (488nm) 2 FITC/ PerCP-Cy5.5
Yellow-Green (561nm) 5 PE/ PE-TR/ PE-Cy5/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ BD LSR Fortessa 2 (5 lasers, 18 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
UV (355nm) 3 BUV395/ BUV496/ BUV737
Violet (405nm) 6 BV421/ BV510/ BV605/ BV650/ BV711/ BV786
Blue (488nm) 2 FITC/ PerCP-Cy5.5
Yellow-Green (561nm) 4 PE/ PE-TR/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ BD LSR X20 (4 lasers, 13 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
Violet (405nm) 3 BV421/ BV510/ BV605
Blue (488nm) 2 FITC/ PerCP-Cy5.5/td>
Green (543nm) 5 PE/ PE-TR/ PE-Cy5/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ Beckman Cytoflex 1 (4 lasers, 13 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes
Violet (405nm) 4 BV421/ BV510/ BV605/ BV650
Blue (488nm) 2 FITC/ PerCP or PerCP-Cy5.5
Red (633nm) 3 APC/ AF700/ APC-Cy7
Yellow 4 PE/ PETR/ PECY5 or PECY5.5/ PEcy7
▸ Beckman Cytoflex 2 (4 lasers, 13 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes
Violet (405nm) 4 BV421/ BV510/ BV605/ BV650
Blue (488nm) 2 FITC/ PerCP or PerCP-Cy5.5
Red (633nm) 3 APC/ AF700/ APC-Cy7
Yellow 4 PE/ PETR/ PECY5 or PECY5.5/ PEcy7

Cell Sorters

Our facility is currently equipped with 3 active high-speed sorters, capable of sorting cells at impressive rates. To view additional details click show more to obtain more regarding specific instruments.

▸ Beckman Mo-Flo Astrios 1 (4 lasers, 14 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
Violet (405nm) 5 BV421/ BV510/ BV605/ BV650/ BV711
Blue (488nm) 2 FITC/ PerCP-Cy5.5
Green (561nm) 4 PE/ PE-TR/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ Beckman Mo-Flo Astrios 2 (3 lasers, 11 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
Violet (405nm) 2 BV421/ BV510
Blue (488nm) 6 FITC/ PE/ PE-TR/ PE-Cy5/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7
▸ BD Aria Fusion (5 lasers, 18 channels)
Laser Color/Wavelength Fluorescent Channels Typical Fluorochromes per channel
UV (355nm) 3 BUV395/ BUV496/ BUV737
Violet (405nm) 6 BV421/ BV510/ BV605/ BV650/ BV711/ BV786
Blue (488nm) 2 FITC/ PerCP-Cy5.5
Yellow-Green (561nm) 4 PE/ PE-TR/ PE-Cy5.5/ PE-Cy7
Red (640nm) 3 APC/ AF700/ APC-Cy7

Fee Table

Service Instruments Internal (Hourly) External Academic
(Hourly)
External Corporate
(Hourly)
Flow Analysis
(unaided)
BD FACS Canto II $30.00 $30.00 $60.00
Flow Analysis
(unaided)
BD Fortessa1, BD Fortessa2, BD Fortessa X20 $40.00 $40.00 $80.00
Flow Analysis
(unaided)
Cytoflex $40.00 $40.00 $80.00
Cell sorting
(peak hours)
BD Fusion, Astrios 1, Astrios2, Astrios3 $75.00 $75.00 $150.00
Cell sorting
(after hours)
BD Fusion, Astrios1, Astrios2, Astrios3 $112.50 $112.50 $225.00
Assisted acquisition, training
(peak hours)
All instruments $75.00 $75.00 $150.00

Questions?

PM Flow, along with all other UHN Core facilities, can be accessed by any researcher, regardless of organization, through our Customer Management site (Stratocore PPMS) here. Create a free account to communicate directly with the facility and submit quotation requests for your work. Please see our brief help documents for Login and Booking .

Scheduling a meeting with us to discuss your research and goals is the best way for us to ensure the optimal technology and equipment is applied to your project. Please contact us for a free consultation by phone at (416) 946-4501 , ext. 5237 (or 16-5237 if calling from within UHN) or by e-mail at: pmflow@uhnresearch.ca

Trained users can book our analyzers online by accessing our Customer Management site (Stratocore PPMS) here. Sorting appointments can be made by contacting our staff directly by phone at (416) 946-4501 , ext. 5237 (or 16-5237 if calling from within UHN) or by e-mail at: pmflow@uhnresearch.ca.

Users can book training online by accessing our Customer Management site (Stratocore PPMS) here. Sorting appointments can be made by contacting our staff directly by phone at (416) 946-4501 , ext. 5237 (or 16-5237 if calling from within UHN) or by e-mail at: pmflow@uhnresearch.ca.

We are located on the 8th floor of the Princess Margaret Cancer Centre (office: 8-605) at 610 University Avenue, Toronto, ON, M5G 2M9.

Our operational and biosafety policies are available to registered users through our Customer Management site (Stratocore PPMS) here.

Recent News

Read all about what we've been up to
picture of moving equipment
June 5, 2020

Instrument Relocation to Improve Safe Access

We have recently relocated some of our analytical instruments in order to allow all instruments to be accessible at any time while maintaining appropriate physical distancing.

Read details here ▸
picture of SBAR allusers poster
June 1, 2020

ATTENTION ALL PMFlow USERS

Things you need to know about PMFlow Research Restart JUNE 1, 2020

Read SBAR details here ▸
picture of SBAR floJo poster
May 20, 2020

ATTENTION ALL FLOW JO USERS!

Flow Jo Analysis Software Migrates to Portal. Current Licenses expire May 31, 2020

Read SBAR details here ▸
picture of calendar November
Oct 25, 2019

Stratocore calendar

The flow cytometry Calcium booking calender is no longer in use. The new Booking-Calendar application from Stratocore is live today!

picture of imaging workshop poster
Oct 25, 2019

New Beckman Coulter Cytoflex Analyzers have arrived

The first of our new Beckman Coulter Cytoflex Analyzers has arrived and will be available to our users in November. This instrument is equipped with 3 lasers (VBR) and will be capable of analyzing 13 fluorescent parameters simultaneously. Feel free to contact us for additional information and to inquire about training.

picture of fcs express bar
Sep 30, 2019

FCS Express 7 Site License Demo

Discover how to save time with your standard flow data analysis using UHN’s FCS Express site license!

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